Composite

Part:BBa_K1124204:Design

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

pLux/tet-mCherry-tetR-aiiA

The construct for Multicellular Analog Clock. This BBa_K1124204 contains only negative feedback. 

AiiA and TetR are the suppressor for negative feedback loops.
 mCherry is the red reporter protein for visualization of the excitation wave (gclock handh).

You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1681
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Source

Constructed from the Distribution

References